|
KMID : 0385020010170010065
|
|
Korean Journal of Laboratory Animal Science
2001 Volume.17 No. 1 p.65 ~ p.73
|
|
|
Effect of Dietary High Fat on Morphology and Active D-Glucose Transport in Rat Small Intestinal Brush Border Membranes
|
|
김용규/Kim, Yong Kyu
조정식/채갑용/강태석/장인석/황진희/이주은/Cho, Jung Sik/Chae, Kab Ryong/Kang, Tae Seok/Jang, In Surk/Hwang, Jin Hee/Lee, Ju Eun
|
|
|
Abstract
|
|
|
|
The current study was designed to determine whether dietary high fat (33.9%) with moderate carbohydrate(27.3%) could influence intestinal function as indicated by changing absorptive cell morphology, enzyme activities and Na^*-dependent D-glucose transport in small intestinal brush border membrane(BBM) of SHR rats. As a result of 7wks feeding trial, quantitative changes in the small intestine were not occurred by dietary high fat. In addition, electromicroscopic examination of the BBM of microvilli in the duodenum showed that BBM height and density were basically identical in both control and HiFat groups. Glucose and protein concentrations and alkaline phosphatase and Na^+/K^+ATPase specific activities in small intestinal mucosa were not significantly altered, although dietary high fat significantly reduced mucosal sucrase activity. The Na^+-dependent glucose transport at various substrate concentrations across intestinal BBM were shown similar magnitude between two groups. Also, the apparent K_m(affinity) and V_(max)(capacity) for the Na^+-dependent glucose transport were not differed between two groups. In conclusion, our finding indicated that dietary high fat with moderate carbohydrate in rat did not noticeably modulate intestinal function such as microvilli morphology and Na^+-dependent glucose transport except mucosal sucrase activity.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|